US 9,957,294 B2Granted

Compositions and methods for analyzing histidine phosphorylation

AssigneesThe Salk Institute for Biological Studies, Sanofi

InventorsMagda Stankova, Fahad Al-Obeidi, Jacques Mauger, Robert A. Binnie, Tony Hunter, Jill Meisenhelder, Stephen Rush Fuhs

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Abstract

A peptide is disclosed of the general structure: Z—W—Y, wherein Z and Y are independently a one to eight amino acid sequence wherein the amino acids are selected from glycine and alanine and W is a non-hydrolyzable pHis analogue. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided are antibodies that specifically bind to a peptide comprising a phosphohistidine (or a non-hydrolyzable pHis analogue) but fail to specifically bind to an identical peptide containing histidine instead of phosphohistidine.

Claims17

Dependent claims

An isolated monoclonal antibody, a humanized form thereof, chimeric form thereof, or an antigen-binding fragment thereof, wherein the monoclonal antibody, humanized form, chimeric form or antigen binding fragment comprises a heavy chain variable domain and a light chain variable domain, wherein:

a) the heavy chain and light chain variable domains are produced by a hybridoma having ATCC accession No. PTA 121685;
or
b) the heavy chain and the light chain variable domains are produced by a hybridoma having ATCC accession No. PTA 121686,
and wherein the monoclonal antibody, humanized form, chimeric form or antigen binding fragment thereof specifically binds a polypeptide comprising a histidine when the histidine is phosphorylated at position N1 of the imidazole ring, but does not specifically bind the polypeptide when the histidine is not phosphorylated at position N1 of the imidazole ring.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 14/485,578Filed 12 Sep 2014 · continuation
Priority: 13 Mar 2012
Published: US 2015/0004173 A11 Jan 2015
Granted: 1 May 2018
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

C07K 7/06
Linear peptides containing only normal peptide links: having 5 to 11 amino acids
C07K 7/08
Linear peptides containing only normal peptide links: having 12 to 20 amino acids
C07K 16/40
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against enzymes
C07K 16/44
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against material not provided for elsewhere , e.g. haptens, metals, DNA, RNA,…

Family

Application61/610,180Us provisional application · filed 13 Mar 2012
Application61/641,607Us provisional application · filed 2 May 2012
PCT/US2013/030917Parent of this continuation · filed 13 Mar 2013
US 2015/0004173 A1Pre-grant publication · published 1 Jan 2015
US 9,957,294 B2This patent

From the US filing history. Foreign family members aren’t listed.