Facile methods for fabricating a uniformly patterned and porous nanofibrous scaffold

A method of producing a nanofibrous polymer membrane sheet comprising:

• providing a syringe;
• providing a solution, the solution comprising a polymer, the polymer being a synthetic or natural polymer, wherein the synthetic polymer being selected from the group consisting of polylactic acid (PLA), polyglycolic acid (PGA), poly-L-lactic acid (PLLA), poly (D, L-lactic-co-glycolic acid) (PLGA), poly (caprolactone) (PCL), aliphatic polyester of poly (valerolactone), poly (hydroxybutyrate) and poly (hydroxy valerate), and wherein the natural polymer is selected from the group consisting of collagen;
• adding the solution comprising the polymer into the syringe;
• providing a voltage source, the voltage source comprising a positive charge and a ground;
• electrically connecting the positive charge to the syringe;
• providing a collector;
• providing a foil;
• attaching the foil around the collector;
• providing a water soluble film;
• mounting the water soluble film on the foil;
• electrically connecting the collector to the ground;
• generating a voltage differential in between the syringe and the collector in response to electrically connecting the positive charge to the syringe and electrically connecting the collector to the ground;
• applying a voltage to the collector and the syringe in response to generating the voltage differential in between the syringe and the collector;
• depositing the solution comprising the polymer on the water soluble film in response to applying the voltage to the collector and the syringe;
• spinning the collector;
• providing water;
• producing a nanofibrous polymer membrane sheet by dissolving the water soluble film in the water in response to depositing the solution comprising the polymer on the water soluble film and spinning the collector;
• and
• isolating the nanofibrous polymer membrane sheet from the foil.