Methods for preparing factor X, activated factor X, inactivated factor X and inactivated factor Xa, and pharmaceutical compositions comprising same
AssigneeBio Products Laboratory Limited
InventorsJoanne Lloyd, Peter Feldman
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Abstract
Methods for preparing Factor X, activated Factor X, inactivated factor X and inactivated factor Xa, compositions comprising Factor X and Factor Xa, inactivated Factor X and inactivated Factor Xa and methods of medical treatment using Factor X, Factor Xa, activated Factor X and inactivated Factor Xa are disclosed. The preparation methods comprise a chromatography step using an immobilised metal ion affinity chromatography substrate.
Claims4
A method of reversing the effect of an anticoagulant in a patient, the method comprising treating the patient with a composition comprising a therapeutically effective amount of factor X or factor Xa and one or more compounds selected from the group consisting of pharmaceutically acceptable diluents, excipients, and stabilizers, treating the patient with a composition comprising a therapeutically effective amount of factor X or factor Xa further comprising preparing the factor X or factor Xa by a process comprising
- a) adsorbing a starting material comprising factor X and prothrombin onto an immobilised metal ion affinity chromatography substrate;
- b) eluting selected adsorbed proteins from the substrate;
- c) monitoring the eluate for commencement of elution of prothrombin and factor X, discarding a first portion of the eluate and collecting a subsequent second portion of the eluate enriched in factor X;
- and
- d) reducing the level of residual prothrombin in the factor X obtained in step c) by anion exchange chromatography.
Dependencies are inferred from the claim text.