Arginine vasopressin pro-hormone as predictive biomarker for diabetes
AssigneesB.R.A.H.M.S GmbH, The General Hospital Corporation
InventorsJoachim Struck, Andreas Bergmann, Olle Melander, Christopher Newton-Cheh, Thomas Wang
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Abstract
Subject of the present invention are assays and in vitro methods for the prediction of the risk of a subject for contracting metabolic syndrome and/or diabetes mellitus and for diagnosing metabolic syndrome, comprising determining the level of arginine vasopressin pro-hormone or fragments thereof in a sample of a subject.
Claims7
A method for diagnosing metabolic syndrome in a subject comprising:
- a. detecting and quantitating the level of arginine vasopressin pro-hormone copeptin fragment in a sample from said patient,
- wherein said detection and quantitation comprises contacting the sample with a diagnostic assay reagent comprising at least one monoclonal antibody or fragment thereof that specifically binds to the arginine vasopressin pro-hormone copeptin fragment at the epitope corresponding to amino acids 132-147 of pre-pro-vasopressin of SEQ ID NO:
- 1, and detecting and quantitating thus-formed complexes of the antibody or fragment thereof and arginine vasopressin pro-hormone copeptin fragment,
- b. detecting and quantitating at least one other clinical and/or laboratory parameter associated with the diagnosis of metabolic syndrome, and
- c. correlating the level of the arginine vasopressin pro-hormone copeptin fragment in conjunction with the at least one other clinical and/or laboratory parameter associated with the diagnosis of metabolic syndrome, with the diagnosis of metabolic syndrome.
An assay for the determination of the copeptin level in a sample, wherein the assay has a total assay imprecision of 20% Coefficient of Variation (CV) at a copeptin concentration below 2.2 pmol/L and a lower than 20% CV at normal range-concentrations above that concentration, and/or 10% CV at a concentration below 9 pmol/l and a lower than 10% CV at normal range-concentrations above that concentration,
- wherein the assay comprises two anti-copeptin antibodies, and
- wherein at least one of the antibodies is a monoclonal antibody or fragment thereof that binds to an epitope comprising amino acids 132-147 of pre-pro-vasopressin of SEQ ID NO:
- 1.
An assay for the determination of the copeptin level in a sample, wherein the assay comprises two anti-copeptin antibodies, wherein at least one of the antibodies is a monoclonal antibody or fragment thereof that binds to an epitope comprising amino acids 132-147 of pre-pro-vasopressin of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.