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US 9,951,386 B2Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims68
A method for single cell processing or analysis, comprising:
- (a) causing a single cell from a plurality of cells to contact one or more antibodies such that an antibody from said one or more antibodies couples to said single cell, wherein said antibody is associated with a first nucleic acid molecule comprising a first barcode sequence;
- (b) co-partitioning said single cell having said antibody coupled thereto with a single bead from a plurality of beads in a droplet, wherein said single bead comprises a second nucleic acid molecule comprising a second barcode sequence;
- and
- (c) generating from said first nucleic acid molecule and said second nucleic acid molecule, a third nucleic acid molecule comprising (i) said first barcode sequence or a complement thereof, and (ii) said second barcode sequence or a complement thereof.
1 more element
A method for single cell processing or analysis, comprising:
- (a) co-partitioning a single cell from a plurality of cells with a single bead from a plurality of beads in a partition, wherein said single cell is coupled to an antibody comprising a first nucleic acid molecule comprising a first barcode sequence, wherein said single bead comprises a second nucleic acid molecule comprising a second barcode sequence;
- and
- (b) in said partition, generating from said first nucleic acid molecule and said second nucleic acid molecule, a third nucleic acid molecule comprising (i) said first barcode sequence or a complement thereof, and (ii) said second barcode sequence or a complement thereof.
Dependencies are inferred from the claim text.