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US 9,951,354 B2Granted
Production of β-phellandrene using genetically engineered cyanobacteria
AssigneeThe Regents of the University of California
InventorsAnastasios Melis, Fiona K. Davies, Hsu-Ching Chen Wintz, Andreas Zurbriggen
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Abstract
The present invention provides methods and compositions for producing β-phellandrene hydrocarbons from cyanobacteria.
Claims14
A method of obtaining β-phellandrene from cyanobacteria, the method comprising:
- culturing a cyanobacteria strain that has been genetically modified to express a heterologous β-phellandrene synthase under conditions in which the β-phellandrene synthase is expressed, wherein the β-phellandrene synthase is encoded by a β-phellandrene synthase polynucleotide having at least 80% nucleic acid sequence identity to SEQ ID NO:3;
- and
- collecting from the surface of the culture medium β-phellandrene hydrocarbons that have spontaneously diffused into the medium from the cyanobacteria across the cell wall.
A method of obtaining β-phellandrene, the method comprising:
- introducing an expression construct comprising a nucleic acid sequence encoding β-phellandrene synthase into a cyanobacteria host cell, wherein the β-phellandrene synthase is encoded by a β-phellandrene synthase polynucleotide having at least 80% nucleic acid sequence identity to SEQ ID NO:3;
- culturing the cyanobacterial host cell under conditions in which the β-phellandrene synthase is expressed;
- and
- collecting β-phellandrene from the surface of the culture medium that are produced by the cyanobacterial host cell and have diffused across the cyanobacterial host cell wall and into the culture media.
1 more element
Dependencies are inferred from the claim text.