Host cells and methods for producing diacid compounds
AssigneeThe Regents of the University of California
InventorsEric J. Steen, Jeffrey L. Fortman, Jeffrey A. Dietrich, Jay D. Keasling
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Abstract
The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.
Claims16
A recombinant cell that produces an omega-hydroxylated fatty acid or a dicarboxylic acid or both from endogenous fatty acids, wherein said cell comprises the enzymes of a Type II fatty acid biosynthesis pathway and a fatty acid omega hydroxylase;
- wherein one or more of said enzymes is encoded by a recombinant nucleic acid in said cell, and the fatty acid omega hydroxylase is one selected from the group consisting of P450 (3P2), P450 (PHP3), P450 BM3 (F87A), P450a1k1 (C. tropicalis;
- C12-C16);
- CPR (C. tropicalis);
- P450 (3P2) (chimeric enzyme;
- C6-C12);
- P450 (pHP3) (Rabbit;
- C6-C12);
- P450 (P. oleovarans;
- C8-C12);
- P450 BM3 (B. megaterium;
- C12-C18);
- CYP86A8 (A. thaliana;
- C12-C18);
- CYP703A1 (Petunia x hybrid;
- C12);
- CYP704B2 (O. sativa ssp japonica;
- C18);
- CYP4V2 (H. sapiens;
- C12-C16);
- CYP4B (H. sapiens;
- C7-C10);
- CYP4A (H. sapiens;
- C 10-C16);
- and CYP4F (H. sapiens;
- C16-C26);
- wherein the recombinant cell is genetically modified to remove a gene encoding YdiO, and the recombinant cell is a bacterial cell.
Dependencies are inferred from the claim text.