Down regulation of the gene expression by means of nucleic acid-loaded virus-like particles
OwnerJens Gruber, Wolfgang Luekeinventors
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions of virus-like particles for the introduction of RNA-interference (RNAi-) inducing molecules into eukaryotic cells and methods for the cell type-specific transduction of a plurality of eukaryotic cells with RNAi-inducing molecules. The present invention furthermore relates to methods for a diagnosis, prevention and/or treatment of diseases or disease states associated with an increased expression rate of at least one endogenous gene, and/or with the undesired expression of at least one endogenous gene and/or foreign nucleic acids, in particular viral nucleic acids.
Claims6
A composition comprising aan isolated virus like particle (VLP), the VLP comprising a modified viral capsid protein 1 from human polyoma JC-virus (JCV-VP1), wherein the modified JCV-VP1 comprises a heterologous nuclear localization signal, and wherein the VLP further comprises an inhibitory RNA (RNAi)-inducing molecule comprising at least one polynucleotide strand having a sequence sufficiently complementary to a target-mRNA to cause the degradation of the target-mRNA.
A composition comprising an isolated virus like particle (VLP), the VLP comprising a modified viral capsid protein 1 from human polyoma JC-virus (JCV-VP1), wherein the modified JCV-VP1 comprises a heterologous nuclear localization signal.
Dependencies are inferred from the claim text.