Biosynthesis of oligosaccharides
AssigneesThe Board of Trustees of the University of Illinois, Seoul National University R&Db Foundation
InventorsWon-Heong Lee, Panchalee Pathanibul, Josh Quarterman, Michael J. Miller, Yong-Su Jin, Jin-Ho Seo
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Abstract
The present invention relates to methods for production of 2′Fucosyllactose by a microbial system comprising a α-1,2 fucosyltransferase (FucT2) polynucleotide and a Guanosine 5′-diphospho-β-L-fucose (GDP-L-fucose) synthesis pathway using lactose as a substrate. Furthermore, the present invention relates to compositions comprising the microbial system.
Claims22
A composition comprising a recombinant microorganism wherein the microorganism expresses a phosphomannomutase (Man B) polynucleotide, a mannose 1-phosphate guanylytransferase (Man C) polynucleotide, a GDP-D-mannose-4,6-dehydratse (Gmd) polynucleotide and a GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase (WcaG) polynucleotide;
- and comprises a heterologous α-1,2 fucosyltransferase polynucleotide, wherein the α-1,2 fucosyltransferase polynucleotide is a Helicobacter pylori polynucleotide, wherein the recombinant microorganism overexpresses α-1,2 fucosyltransferase, and wherein 2′-fucosyllactose can be produced at an amount of 2.5 or more g/L.
Dependencies are inferred from the claim text.