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US 9,943,333 B2Granted
Sperm activating agent and activating method using same
AssigneesNational University Corporation, Hamamatsu University School of Medicine
InventorsKazuhiro Sugihara, Michiko Fukuda
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Abstract
The present invention relates to an agent for activating mammalian sperm and a method for activating sperm by use of the activating agent. More specifically, the present invention relates to a sperm activating agent containing endo-β-galactosidase and/or FGF, which accelerate motility of mammalian sperm, for use in in-vitro fertilization and artificial insemination by husband and a sperm activating method by adding endo-β-galactosidase and/or FGF.
Claims8
A method of activating a sperm comprising:
- (a) incubating the sperm in the presence of endo-β-galactosidase and FGF for up to 60 minutes to activate the sperm;
- or
- (b) incubating the sperm, which has been endo-β-galactosidase treated, in the presence of FGF for up to 60 minutes to activate the sperm.
Dependencies are inferred from the claim text.