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US 9,939,373 B2Granted
Interactive variable pathlength device
AssigneeC Technologies AB
InventorsMark Salemo, I-Tsung Shih, Craig Harrison
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Abstract
This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.
Claims13
A method of determining the concentration of a compound in a solution comprising:
- (a) taking a first absorbance reading at a predetermined wavelength of the compound in the solution at a first pathlength;
- (b) changing the first pathlength by an increment to provide a second pathlength and taking a second absorbance reading at the predetermined wavelength;
- (c) repeating steps (a) and (b) one or more times;
- (d) generating a regression line from the absorbance values such that a slope of the regression line is obtained;
- and
- (e) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the compound in the solution.
3 more elements
A method of determining the extinction coefficient of a compound in a solution at a wavelength where the extinction coefficient is not known comprising:
- (a) taking a first absorbance reading of the solution at a first predetermined wavelength where the extinction coefficient is known and a second predetermined wavelength where the extinction coefficient is not known at a given pathlength;
- (b) repeating step (c) one or more times to determine the ratio of the absorbance to the path length at the first wavelength and the second wavelength;
- (c) calculate the extinction coefficient at the second wavelength from the ratios of the absorbance to path length at the first and second wavelengths and the extinction coefficient for the first wavelength.
A method of determining the concentration of a compound in a solution comprising:
- (a) taking an first absorbance spectrum of the compound in the solution at a first pathlength;
- (b) changing the first pathlength by an increment to provide a second pathlength and taking a second absorbance spectrum reading at the predetermined wavelength;
- (c) repeating steps (a) and (b) one or more times;
- (d) generating a regression line from the absorbance values at a given wavelength such that a slope of the regression line is obtained;
- and
- (e) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the compound in the solution.
3 more elements
Dependencies are inferred from the claim text.