Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

A method of differentiating human pluripotent stem cells intoEMHlin−KDR+APLNR+PDGFRalpha+ primitive mesoderm cells, comprising the steps of:

• (a) providing human pluripotent stem cells;
• and
• (b) exposing the stem cells to a xenogen-free and serum albumin-free mixture comprising components of about 25 ng/ml to about 50 ng/ml FGF2, BMP4 of at least 50 ng/ml to about 250 mg/ml, Activin A of less than 15 ng/ml, and about 1 mM to about 2 mM LiCl under hypoxic conditions for a period of about two days to form a population of EMHlin−KDR+APLNR+PDGFRalpha+primitive mesoderm cells with mesenchymoangioblast potential without the formation of embryoid bodies or coculture with stromal cell lines.