Glycosylated polypeptide and drug composition containing said polypeptide
AssigneeGlytech, Inc.
InventorsHirofumi Ochiai, Taiji Shimoda, Kazuhiro Fukae, Masatoshi Maeda, Kazuyuki Ishii, Kenta Yoshida, Katsunari Tezuka, Keisuke Tazuru
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Abstract
[Problem] To provide a glycosylated polypeptide having an affinity to somatostatin receptors, and, compared to somatostatins, having improved in-blood stability. [Solution] The glycosylated polypeptide is characterized by at least one amino acid in a somatostatin or an analog thereof being replaced with a glycosylated amino acid.
Claims15
A glycosylated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (A) a SRIF14 consisting of the amino acid sequence of SEQ ID NO:1;
- (B) a polypeptide having one amino acid deleted from, substituted with, or added to SRIF14, SRIF14 consisting of the amino acid sequence of SEQ ID NO:
- 1;
- and
- (C) a polypeptide having 90% or more homology with SRIF14 consisting of the amino acid sequence of SEQ ID NO:1;
- wherein said glycosylated polypeptide further comprises N amino acids at the N-terminal side, wherein N is an integer from 1 or more to 20 or less;
- and/or
- said glycosylated polypeptide further comprises M amino acids at the C-terminal side, wherein M is an integer from 1 or more to 6 or less;
- wherein at least one amino acid is substituted with a glycosylated amino acid ad at least one of the at least one amino acid substituted with said glycosylated amino acid(s) is present in any of said N amino acids or said M amino ids and the glycosylated polypeptide has affinity towards somatostatin receptors.
A glycosylated polypeptide comprising amino acid sequence selected from the group consisting of:
- (A) a SRIF28 consisting of the amino acid sequence of SEQ ID NO:2;
- (B) a polypeptide having one or two amino acids deleted from, substituted with, or added to SRIF28, SRIF28 consisting of the amino acid sequence of SEQ ID NO:2;
- (C) a polypeptide having 90% or more homology with SRIF28 consisting of the amino acid sequence of SEQ ID NO:2;
- (D) a polypeptide further comprising J amino acids at the N-terminal side of (A)-(C), wherein J is an Integer from 1 or more to 6 or less;
- and
- (E) a polypeptide further comprising K amino acids at the C-terminal side of (A)-(C), wherein K is an integer from 1 or more to 6 or less;
- wherein at least one amino acid of said polypeptide (A) to (E) is substituted with a glycosylated amino acid and
- said at least one amino acid substituted with said glycosylated amino acid is an amino acid selected from the group consisting of the amino acid corresponding to position 1, the amino acid corresponding to position 5, the amino acid corresponding to position 9, the amino acid corresponding to position 12, the amino acid corresponding to position 13, the amino acid corresponding to position 14, the amino acid corresponding to position 19 of SRIF28, and any combination thereof;
- said at least one amino acid substituted with said glycosylated amino acid is present in said J amino acids;
- and/or
- said at least one amino acid substituted with said glycosylated amino acid is resent in said K amino aids, and
- the polypeptide ha affinity towards somatostatin receptors.
Dependencies are inferred from the claim text.