# US 9,933,418 B2 · Multi-unit plate for electroblotting and immunoblot analysis

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- Number: US 9,933,418 B2
- Document ID: US9933418B2
- Status: Granted
- Kind: B2
- Application number: 14/721,205
- Filed: 2015-05-26
- Published: 2016-12-01
- Granted: 2018-04-03
- Earliest claimed: 2015-05-26
- Assignees: Quanticision Diagnostics Inc.
- Applicants: Jiandi Zhang
- Inventors: Jiandi Zhang
- Web page: https://patentgrep.com/patent/US9933418B2

## CPC

- B01L 3/5085: {for multiple samples, e.g. microtitration plates}
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- B01L 2300/0609: Holders integrated in container to position an object
- B01L 2300/069: Absorbents; Gels to retain a fluid
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/163: Biocompatibility
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

This invention is provided for multi-unit plate with at least one unit comprised of a membrane, preferably nitrocellulose or PVDF membrane, associated with a supporting structure to allow for electroblotting of the membrane. This multi-unit plate is suited for high throughput immunoblot analysis including Zestern analysis.

## Claims

### Claim 1

A multi-unit plate for immunoblot analysis, comprising:
a plate having a plurality of openings; and
a plurality of protrusive members protruding away from the plate,
wherein one or more of the plurality of protrusive members comprises an elongated linking member having a first end attached to the plate and a second end that provides a support structure having a membrane affixed to the support structure,
wherein the support structure comprises a hollow center portion and a rim surrounding the hollow center portion, and
wherein the membrane is affixed to the rim of the support structure.

### Claim 2 (depends on 1)

The multi-unit plate of claim 1, wherein the elongated linking member is a rod and the rim in the support structure is a ring surrounding the hollow center portion and the membrane is affixed to the ring.

### Claim 3 (depends on 2)

The multi-unit plate of claim 2, wherein the membrane is affixed to the side of ring disposed away from the plate.

### Claim 4 (depends on 1)

The multi-unit plate of claim 1, wherein the elongated linking member is a tubular member having the first end attached to the plate and the second end having the rim, wherein the rim has one or more openings therein so that the membrane is separated from the tubular member at a hollow center portion of the tubular member and at the one or more openings in the rim of the second end of the tubular member.

### Claim 5 (depends on 1)

The multi-unit plate of claim 1, wherein the membrane is a nitrocellulose or PVDF membrane.

### Claim 6 (depends on 1)

The multi-unit plate of claim 1, wherein the support structure is made from a material selected from the group consisting of ceramics, elastomers, epoxies, glasses, glass-ceramics, plastics, polycarbonates, polydimethylsiloxane, polyethylenterephatalate glycol, polymers, polymethyl methacrylate, polystyrene, polyurethane, polyvinyl chloride, rubber, silicon, silicon oxide, and silicon rubber.

### Claim 7 (depends on 1)

The multi-unit plate of claim 1, wherein the protrusive member comprises more than one elongated linking members.

### Claim 8 (depends on 1)

The multi-unit plate of claim 1, wherein a number of the protrusive member is 6, 24, 96, 384, or 1536.

### Claim 9 (depends on 1)

A method for immunoblot analysis, comprises:
loading a sample containing a protein into one or more protrusive members of the multi-unit plate of claim 1;
inserting the multi-unit plate into a multi-well plate so that each protrusive member of the multi-unit plate is placed inside a well of the multi-well plate;
subjecting the membrane in one or more of the plurality of protrusive members successively to blocking with a blocking buffer, incubation with a primary antibody, washing, incubation with a secondary antibody, and washing to obtain one or more processed membranes; applying a detection reagent to the one or more processed membranes; and
placing the multi-unit plate in a microplate reader.

### Claim 10 (depends on 9)

The method of claim 9, wherein the sample is selected from the group consisting of a chemical molecule, a peptide molecule, a protein molecule, an RNA molecule, a DNA molecule, a traditional antibody, a recombinant antibody or fragment thereof, a cell, a virus particle, or mixtures thereof.

### Claim 11 (depends on 1)

A device for immunoblot analysis, comprises a multi-unit plate of claim 1 and a multi-well plate, wherein the multi-well plate comprises a plurality of wells that receive the plurality of protrusive members of the multi-unit plate.