Recombinant microorganism having enhanced 1,3-propanediol producing ability and method for producing 1,3-propanediol using the same
AssigneeGs Caltex Corporation
InventorsTaek-Ho Yang, Chelladural Rathnasi
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Abstract
The present invention relates to a recombinant microorganism for producing 1,3-propanediol, wherein a pathway converting pyruvate into 2,3-butanediol is inhibited in a microorganism having a pyruvate and acetyl CoA biosynthetic pathway. In addition, the present invention relates to a method for producing 1,3-propanediol by using the recombinant microorganism.
Claims9
A recombinant Klebsiella for producing 1,3-propanediol, wherein
- a pathway for converting pyruvate into α-acetolactate catalyzed by α-acetolactate synthase, a pathway for converting α-acetolactate into acetoin catalyzed by α-acetolactate decarboxylase, a pathway for converting acetoin into 2,3-butanediol catalyzed by acetoin reductase, a pathway for converting pyruvate into lactate catalyzed by lactate dehydrogenase, and a pathway for converting pyruvate into acetyl-CoA catalyzed by pyruvate-formate lyase are suppressed in recombinant Klebsiella, and
- the recombinant Klebsiella has no ability to produce succinic acid.
A recombinant Klebsiella for producing 1,3-propanediol, wherein a gene having the nucleotide sequence of SEQ ID NO:
- 1, a gene having the nucleotide sequence of SEQ ID NO:
- 9 and a gene having the nucleotide sequence of SEQ ID NO:
- 33 are suppressed in the recombinant Klebsiella.
A recombinant Klebsiella for producing 1,3-propanediol, wherein a gene encoding lactate dehydrogenase, a gene encoding pyruvate-formate lyase, a gene encoding transcription activation factor, a gene encoding α-acetolactate decarboxylase, a gene encoding α-acetolactate synthase, and a gene encoding acetoin reductase are suppressed in the recombinant Klebsiella.
Dependencies are inferred from the claim text.