Recombinant microorganism producing 1,3-diaminopropane and method for producing 1,3-diaminopropane using the same
AssigneeKorea Advanced Institute of Science and Technology
InventorsSang Yup Lee, Tong Un Chae
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Abstract
The present disclosure relates to a recombinant microorganism producing 1,3-diaminopropane, and a method for producing 1,3-diaminopropane using the same, and specifically, to a recombinant microorganism producing 1,3-diaminopropane into which genes encoding an enzyme involved in a metabolic pathway of 1,3-diaminopropane, dat and ddc, are introduced, and a method for producing 1,3-diaminopropane using the same. When the recombinant microorganism producing the 1,3-diaminopropane according to the present disclosure is used, 1,3-diaminopropane may be mass-produced to be industrially useful in various fields such as pharmaceutical products, agricultural products, fibers for clothing, etc.
Claims13
A recombinant microorganism producing 1,3-diaminopropane in which a gene encoding 2-ketoglutarate 4-aminotransferase (dat) and a gene encoding 2,4-diaminobutanoate decarboxylase (ddc) are introduced into a microorganism having an ability of producing aspartate-4-semialdehyde (ASA),
- wherein the recombinant microorganism comprises further mutation of base sequence of a gene encoding aspartokinase I and/or a gene encoding aspartokinase III to remove feedback inhibiting production of 1,3-diaminopropane,
- wherein the mutation of base sequence of a gene encoding aspartokinase I is the substitution of C of 1034th base sequence with T, and the mutation of base sequence of a gene encoding aspartokinase III is the substitution of C of 1055th base sequence with T,
- wherein the recombinant microorganism producing the 1,3-diaminopropane comprises further variation selected from the group consisting of:
- (i) over-expression of a gene encoding aspartate aminotransferase;
- and
- (ii) deletion of a gene encoding 6-phosphofructokinase I.
Dependencies are inferred from the claim text.