Reduction of lipase activity in product formulations
AssigneeUniversity of Delaware
InventorsKelvin Lee, Abraham Lenhoff, Kristin Valente, Nick Levy, Yatin Gokarn
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Abstract
The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
Claims4
A non-naturally occurring host cell comprising a nucleic acid sequence encoding recombinant protein, wherein the host cell expresses the recombinant protein, wherein the host cell expresses an interfering RNA specific for an endogenous lipoprotein lipase, whereby the production of the endogenous lipoprotein lipase by the host cell is reduced.
Dependencies are inferred from the claim text.