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US 9,932,570 B2Granted
Anthrax lethal factor substrates and methods of use thereof
AssigneeList Biological Laboratories, Inc.
InventorNancy Shine
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Abstract
Disclosed herein is a peptide substrate that is cleaved specifically by anthrax lethal factor (LF), and assays for detecting the presence of anthrax lethal factor in a sample using the peptide substrate. In some cases, the sample may be obtained from an individual at an early stage of an anthrax infection. Kits that include the peptide substrate and that find use in carrying out the assays are also disclosed.
Claims24
An anthrax lethal factor-specific peptide substrate comprising an amino acid sequence of the formula X1-X2-X3-X4-X5-X6-X7* (SEQ ID NO:8), wherein
- X1 is either present or absent, and when present, is Arg;
- X2 is Arg;
- X3 is Lys;
- X4 is Lys;
- X5 is Val;
- X6 is Tyr;
- and
- X7* has the formula X7—Z, where
- X7 is Pro, and
- Z is a detectable label covalently linked by an amide linkage or an ester linkage to an alpha carboxyl group of X7, and
- wherein at least one of X1, X2, X3 and X4, is a D isomer of the amino acid.
8 more elements
Dependencies are inferred from the claim text.