Back to results
US 9,932,567 B1Granted
Mutant reverse transcriptase
AssigneeNew England Biolabs, Inc.
InventorsYan Xu, Jennifer Ong, Shengxi Guan, Nicole Nichols
Life of this patent
Estimating the term…
Abstract
A mutant MMLV reverse transcriptase that may have an improvement in one or more properties is provided. For example, the present reverse transcriptase is believed to be more efficient relative to other commercially available MMLV reverse transcriptase variants, particularly for templates with a higher GC content.
Claims14
A method comprising:
- (a) obtaining a reaction mix by combining a primer, an RNA template and a reverse transcriptase, wherein the reverse transcriptase comprises:
- at least 300 contiguous amino acids of SEQ ID NO:
- 1;
- and
- (b) incubating the reaction mix to produce cDNA copied from the RNA template.
2 more elements
A method comprising:
- (a) obtaining a reaction mix by combining a primer, an RNA template and a reverse transcriptase, wherein the reverse transcriptase comprises:
- (i) amino acids 24-335 of SEQ ID NO:
- 1 and (ii) an amino acid sequence that is at least 90% identical to amino acids 1-286 of SEQ ID NO:
- 2 wherein the amino acid sequence of (i) is N-terminal to the amino acid sequence of (ii);
- and
- (b) incubating the reaction mix to produce cDNA copied from the RNA template.
3 more elements
Dependencies are inferred from the claim text.