Recombinant isfahan viral vectors
AssigneesBoard of Regents, The University of Texas System, Profectus Biosciences, Inc.
InventorsDemetrius Matassov, Rodion V. Gorchakov, Stefan Hamm, Rebecca Nowak, Robert L. Seymour, John H. Eldridge, Robert B. Tesh, David K. Clarke, Theresa E. Latham, Scott Weaver, Farooq Nasar
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Abstract
Certain embodiments are directed to recombinant vesiculovirus encoding a heterologous polynucleotide and methods of using the same.
Claims25
A recombinant replication competent Isfahan virus comprising an N protein gene, a P protein gene, an M protein gene, a G protein gene, and an L protein gene;
- and further comprising a heterologous polynucleotide sequence encoding a heterologous polypeptide.
An immunogenic composition comprising a recombinant replication competent Isfahan virus comprising an N protein gene, a P protein gene, an M protein gene, a G protein gene, and an L protein gene;
- and further comprising a heterologous polynucleotide sequence, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide;
- and a pharmaceutically acceptable diluent, excipient or carrier.
An immunization kit for inducing an antigen-specific immune response in a mammalian subject, said kit comprising:
- (a) a priming composition comprising a recombinant replication competent Isfahan virus encoding an N protein gene, a P protein gene, an M protein gene, a G protein gene, an L protein gene, and a heterologous polynucleotide sequence, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide, wherein said heterologous polynucleotide sequence (i) is flanked by a transcription start signal and a transcription stop signal, and (ii) encodes a heterologous polypeptide;
- and a pharmaceutically acceptable diluent, excipient or carrier;
- and
- (b) a boosting composition comprising a recombinant replication competent vesicular stomatitis virus encoding an N protein gene, a P protein gene, an M protein gene, a G protein gene, an L protein gene, and a heterologous polynucleotide sequence, wherein said heterologous polynucleotide sequence (i) is flanked by a transcription start signal and a transcription stop signal, and (ii) encodes a heterologous polypeptide;
- and a pharmaceutically acceptable diluent, excipient or carrier.
An immunization kit for inducing an antigen-specific immune response in a mammalian subject, said kit comprising:
- (a) a priming composition comprising a recombinant replication competent vesicular stomatitis virus (VSV) encoding an N protein gene, a P protein gene, an M protein gene, a G protein gene, an L protein gene, and a heterologous polynucleotide sequence, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide, wherein said heterologous polynucleotide sequence (i) is flanked by a transcription start signal and a transcription stop signal, and (ii) encodes a heterologous polypeptide;
- and a pharmaceutically acceptable diluent, excipient or carrier;
- and
- (b) a boosting composition comprising a recombinant replication competent Isfahan virus (ISFV) encoding an N protein gene, a P protein gene, an M protein gene, a G protein gene, an L protein gene, and a heterologous polynucleotide sequence, wherein said heterologous polynucleotide sequence encodes a heterologous polypeptide, wherein said heterologous polynucleotide sequence (i) is flanked by a transcription start signal and a transcription stop signal, and (ii) encodes a heterologous polypeptide;
- and a pharmaceutically acceptable diluent, excipient or carrier.
A recombinant replication competent Isfahan virus comprising an N protein gene, a P protein gene, an M protein gene, a heterologous viral surface protein gene(s), and an L protein gene.
An oncolytic viral composition comprising a recombinant replication competent Isfahan virus comprising an N protein gene, a P protein gene, an M protein gene, a G protein gene, and an L protein gene, wherein said Isfahan virus is used as an anti-cancer therapeutic.
Dependencies are inferred from the claim text.