System and methods for preparation of adipose-derived stem cells

A system for processing tissue in a closed pathway for maintaining sterility comprising:

• a first centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume, the first centrifuge tube further comprising:
• a tissue inlet fitting,
• a suction fitting,
• at least one processing material inlet fitting,
• at least two extraction fittings;
• an extraction tube releasably affixed to each of the at least two extraction fittings that extends a distance within the interior volume towards the apex;
• and
• a screen disposed within the interior volume of the body;
• and,
• a second centrifuge tube comprising an essentially cylindrical body having a top, an inverse conical apex, and an interior volume, the second centrifuge tube further comprising a tissue inlet fitting, a suction fitting, an extraction fitting, and an extraction tube releasably affixed to the extraction fitting that extends a distance within the interior volume towards the apex, wherein the second centrifuge tube is releasably connected via the tissue inlet fitting to one of the at least two extraction fittings of the first centrifuge tube.

A multiple unit enabled system for processing tissue in a closed pathway for maintaining sterility comprising:

• a centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume and further comprising:
• a tissue inlet fitting,
• a suction fitting,
• at least one processing material inlet fitting,
• at least two extraction fittings,
• an extraction tube releasably affixed to each of the at least two extraction fittings that extends a distance within the interior volume towards the apex, and
• a screen disposed within the interior volume of the body;
• and,
• a container having an inlet fitting, at least one extraction fitting, and a suction fitting wherein the inlet port can be releasably connected to one of the at least two extraction fittings of the centrifuge tube for the sterile transfer of material contained with the closed system.