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US 9,930,883 B2Granted
Compositions and methods for preservation of materials in the amorphous state
AssigneeUniversity of North Carollina Charlotte
InventorsGloria Elliott, Babak Bagheri
Life of this patent
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Abstract
The present disclosure provides methods and compositions including a sugar and a choline hydrogen phosphate salt for preservation and stabilization of materials in the amorphous state. The compositions and methods suppress the formation of crystals in materials in the amorphous state.
Claims22
A method for preservation of a biomaterial comprising:
- combining the biomaterial with a preservative composition comprising a sugar or sugar alcohol and a salt comprising choline hydrogen phosphate (HPO4−2) to form a mixture, wherein the salt in the preservative composition comprises 50% or more of HPO4−2 and 50% or less of choline dihydrogen phosphate (H2PO4−);
- and
- converting the preservative composition around, within, or around and within the biomaterial to an amorphous state.
A method of suppressing crystallization of a composition in an amorphous state comprising a sugar or sugar alcohol, the method comprising:
- combining the sugar or sugar alcohol with a salt comprising choline hydrogen phosphate (HPO4−2) in an amount effective to increase the amount of time to formation of crystals in the composition when in the amorphous state as compared to a composition in the amorphous state made without the choline hydrogen phosphate or made with a greater amount of choline dihydrogen phosphate (H2PO4−) than choline hydrogen phosphate.
A preservative composition comprising:
- a sugar or sugar alcohol and
- a salt comprising choline hydrogen phosphate (HPO4−2), wherein the choline hydrogen phosphate comprises 50% or more of the salt,
- the composition having a salt:sugar ratio effective to suppress the formation of crystals in the composition in the amorphous state such that the amount of time to formation of crystals in the presence of moisture is increased over the amount of time to crystal formation in a comparative composition that is the same as the preservative composition except for having a greater amount of choline dihydrogen phosphate (H2PO4−) than the salt of the preservative composition.
A composition comprising:
- a living biomaterial, and
- a preservative composition comprising a sugar or sugar alcohol and a salt comprising 50% or more of choline hydrogen phosphate (HPO4−2) and 50% or less of choline dihydrogen phosphate (H2PO4−),
- wherein the preservative composition is in an amorphous state and wherein the amount of time to formation of crystals in the presence of moisture is increased over the amount of time to crystal formation in a comparative composition comprising the biomaterial without the salt in the preservative composition comprising 50% or more of choline hydrogen phosphate.
Dependencies are inferred from the claim text.