NAD analogs and methods of using said NAD analogs in determining ribosylation of proteins with PARP mutants
AssigneesBoard of Regents, The University of Texas System, Biolog Life Science Institute Forshungslabor und Biochemica-Vertrieb GmbH, Cornell University
InventorsW. Lee Kraus, Bryan A. Gibson, Frank Schwede, Hening Lin
Life of this patent
Estimating the term…
Abstract
The present disclosure provides NAD analogs of the formula: wherein R1, n, and X1 are defined herein, useful for the identification of PARP target proteins and the amino acid sequence wherein the ribose group is attached to the target protein. Also, provided herein are methods of identifying target protein and amino acid sequences. Additionally, the present disclosure provides methods of identifying DNA sequences associated with specific chromatin proteins.
Claims22
A compound of the formula:
- wherein:
- X1 is -A1-Y1, wherein:
- A1 is —O—, or —S;
- Y1 is cycloalkyl(C≤12), alkynyl(C≤12), aryl(C≤12), aralkyl(C≤12), heteroaryl(C≤12), acyl(C≤12), alkoxy(C≤12), wherein any of these groups is optionally substituted;
- or
- X1 is -A2-L1-Y2, wherein:
- A2 is —O—, or —S;
- L1 is a linker which comprises a 1,2,3-triazole;
- and
- Y2 is a solid support, a biotin, a fluorophore, a protein, an enzyme, a DNA sequence, or an antibody;
- R1 is amino, aminosulfonyl, carboxy, cyano, halo, hydroxy, hydroxysulfonyl, hydroxyamino, mercapto, nitro, or
- alkyl(C≤6), alkoxy(C≤6), acyloxy(C≤6), alkylamino(C≤6), dialkylamino(C≤6), amido(C≤6), wherein any of these groups is optionally substituted;
- and
- n is 0, 1, 2, 3, or 4;
- or a salt, a reduced form, or tautomer thereof.
Dependencies are inferred from the claim text.