Methods, reagents and cells for biosynthesizing compounds
AssigneeInvista North America S.A.R.L.
InventorsNadia Fatma Kadi, Mariusz Kamionka, Alexander Brett Foster, Alex Van Eck Conradie, Adriana Leonora Botes
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Abstract
This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as malonyl-CoA or malonyl-[acp].
Claims23
A method of biosynthesizing glutarate methyl ester in a recombinant host comprising at least one exogenous nucleic acid encoding a polypeptide having malonyl-CoA O-methyltransferase activity and a polypeptide having thioesterase activity, the method comprising enzymatically converting at least one of malonyl-[acp] and malonyl-CoA to glutarate methyl ester in said host, wherein said enzymatic conversion occurs in the host using a polypeptide having malonyl-CoA O-methyltransferase activity, a polypeptide having thioesterase activity, or a combination thereof;
- wherein malonyl-[acp] is enzymatically converted to malonyl-[acp] methyl ester using said at least one polypeptide having malonyl-CoA O-methyltransferase activity, wherein said polypeptide having malonyl-CoA O-methyltransferase activity has at least 85% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 21 and is capable of enzymatically converting malonyl-[acp] to malonyl-[acp] methyl ester, the method optionally further comprising enzymatically converting glutarate methyl ester to glutarate semialdehyde methyl ester in said host using at least one polypeptide having carboxylate reductase activity, wherein the polypeptide having carboxylate reductase activity has at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 and is capable of enzymatically converting glutarate methyl ester to glutarate semialdehyde methyl ester.
Dependencies are inferred from the claim text.