Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same
AssigneesLG Chem, Ltd., Korea Advanced Institute of Science and Technology
InventorsSi Jae Park, Sang Hyun Lee, Sang Yup Lee, Eun Jeong Lee
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Abstract
A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
Claims24
An isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate, and 4-hydroxybutyrate into 1,4-butanediol, in a Lumen bacteria capable of producing succinate,
- wherein the Lumen bacteria have inactive genes encoding lactate dehydrogenase (ldhA) and pyruvate-formate lyase (pfl), and produce succinate in high concentration without substantial production of other organic acids in an anaerobic condition, and
- wherein the gene encoding the enzyme converting succinate into 4-hydroxybutyrate is selected from the group consisting of genes encoding succinyl-CoA transferase (Cat1), succinate semialdehyde dehydrogenase (SucD), 4-hydroxybutyrate dehydrogenase (4hbD) and 4-hydroxybutyrate dehydrogenase (GHB).
An isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying:
- a gene encoding succinyl-CoA transferase (Cat1);
- a gene encoding succinate semialdehyde dehydrogenase (SucD);
- a gene encoding 4-hydroxybutyrate dehydrogenase (4hbD) or 4-hydroxybutyrate dehydrogenase (GHB);
- a gene encoding 4-hydroxybutyrate-CoA transferase, or a gene encoding phosphotransbutyrylase (Ptb) and a gene encoding butyryl kinase (Buk);
- and
- a gene encoding butyl-CoA dehydrogenase,
- in a Lumen bacteria capable of producing succinate,
- wherein the Lumen bacteria have inactive genes encoding lactate dehydrogenase (ldhA) and pyruvate-formate lyase (pfl), and produce succinate in high concentration without substantial production of other organic acids in an anaerobic condition.
Dependencies are inferred from the claim text.