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US 9,920,309 B2Granted
Enzyme-composition for hydrolyzing biomass
AssigneeClariant International Ltd.
InventorsChristoph Reisinger, Lutz Roecher, Michael Kraus, Christian Gamauf
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Abstract
The present invention is directed to an enzyme-composition for hydrolyzing biomass containing comprising at least one cellulase, at least one hemicellulases and/or at least one pectinases. In a further aspect, the present invention is directed to a process for hydrolyzing biomass implementing this enzyme-composition and the use of the enzyme-composition for hydrolyzing biomass.
Claims5
An enzyme composition comprising:
- at least one cellulase,
- at least one hemicellulase and
- at least one pectinase,
- wherein said at least one cellulase comprises at least one enzyme selected from the group consisting of cellobiohydrolases and endo-1,4-β-glucanase;
- wherein said at least one hemicellulase comprises an Arabinan endo-1,5-alpha-L-arabinosidase (EC 3.2.1.99) and the arabinan degrading activity of the Arabinan endo-1,5-alpha-L-arabinosidase (EC 3.2.1.99) is at least 8 times as much as the cellulose degrading activity of the at least one cellulase;
- wherein said at least one pectinase comprises at least one enzyme selected from the group consisting of pectinesterases, polygalacturonases, pectinlyases, mannosidases and rhamnogalacturonases;
- wherein the arabinan degrading activity of the Arabinan endo-1,5-alpha-L-arabinosidase (EC 3.2.1.99) is at least 10 times as much as the pectin degrading activity of the at least one pectinase;
- wherein said at least one cellulase, said one least one hemicellulase, and said at least one pectinase are in a non-naturally occurring ratio;
- and
- wherein said enzyme-composition provides improved arabinose yield and liquefaction.
7 more elements
Dependencies are inferred from the claim text.