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US 9,919,078 B1Granted
Repair of tympanic membrane using human birth tissue material
AssigneeBioDlogics, LLC
InventorTimothy R. Brahm
Life of this patent
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Abstract
A tympanic construct fabricated from at least one amniotic membrane, at least one chorionic membrane, or at least one amniotic membrane and at least one chorionic membrane obtained from human birth tissue is provided. Methods of preparing a tympanic construct, methods of repairing tympanic membrane defects and surgical sites, as well as kits for the same are also provided.
Claims12
A method of preparing a membrane for a tympanic construct,
- comprising the steps of:
- (a) obtaining amniotic membrane, chorionic membrane, or both amniotic and chorionic membrane from a seronegative, healthy human via Cesarean section or vaginal delivery;
- (b) immersing the membrane in a basin containing a sterile saline solution;
- (c) agitating the basin to liberate excess blood and fluids from the membrane;
- (d) rinsing the membrane with a sterile saline solution;
- (e) covering the membrane with a substrate on both the fetal membrane side and the maternal membrane side;
- (f) immersing the membrane in an alcohol composition of 90% to 100% ethanol for a period of from 24 hours to 384 hours;
- (g) removing the substrate from both the fetal membrane side and the maternal membrane side;
- (h) spreading the membrane on a flat, dry and sterile surface;
- (i) allowing the membrane to air dry completely at ambient temperature for a period of up to three hours;
- (j) cutting the membrane to a predetermined size;
- and
- (k) placing the fetal side of the membrane directly onto a pre-cut substrate to form a tympanic construct.
10 more elements
Dependencies are inferred from the claim text.