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US 9,915,663 B2Granted
Thyroglobulin quantitation by mass spectroscopy
AssigneeQuest Diagnostics Investments Incorporated
InventorsYanni Zhang, Nigel J. Clarke, Richard E. Reitz
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Abstract
Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.
Claims11
A method for determining the amount of thyroglobulin in a test sample, comprising:
- (a) digesting thyroglobulin (Tg) and an added isotopically labeled thyroglobulin peptide standard in said test sample to form Tg peptides and Tg peptide standard products, wherein the thyroglobulin peptide standard comprises SEQ ID NO:
- 2 (KVPESKVIFDANAPVAVRSKVPDS);
- (b) ionizing the Tg peptide and the isotopically labeled internal standard;
- and
- (c) quantifying the amount of the Tg peptide ion from step (b) by mass spectrometry;
- wherein the amount of the Tg peptide ion detected is related to the amount of thyroglobulin in said test sample.
3 more elements
Dependencies are inferred from the claim text.