Aldolase, aldolase mutant, and method and composition for producing tagatose by using same
AssigneeSamyang Corporation
InventorsDeok-Kun Oh, Seung-Hye Hong, Seon-Hwa Lee
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Abstract
This disclosure relates to aldolase, an aldolase mutant, and a method and a composition for producing tagatose by using the same. The feature of the disclosure is environment-friendly due to the use of an enzyme acquired from microorganisms, requires only a simple process of enzyme immobilization, uses a low-cost substrate in a substrate compared with a conventional method for producing tagatose and has a remarkably high yield, thereby greatly reducing production costs and maximizing production effects.
Claims2
A mutant enzyme capable of converting fructose 6-phosphate into tagatose-6 phosphate, the mutant enzyme consisting of SEQ ID NO:
- 1 with one or more substitution selected from the group consisting of:
- i) a substitution of arginine residue with glutamine at a position corresponding to position 332,
- ii) a substitution of glutamine residue with alanine at a position corresponding to position 314, and
- iii) a substitution of histidine residue with alanine at a position corresponding to position 227.
Dependencies are inferred from the claim text.