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US 9,909,179 B2Granted
Single-cell nucleic acid analysis
AssigneeFluidigm Corporation
InventorsAmy Hamilton, Min Lin, Alain Mir, Martin Pieprzyk
Life of this patent
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Abstract
The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
Claims20
A method for analyzing nucleic acids from a single cell, said method comprising:
- (a) preamplifying DNA obtained from a single cell using a plurality of target-specific primers to produce a preamplification reaction mixture comprising a plurality of amplicons specific for a plurality of target nucleic acids;
- (b) distributing the plurality of amplicons within a device comprising separate chambers;
- (c) amplifying and detecting the plurality of amplicons, wherein different amplicons are amplified and detected in separate chambers.
Dependencies are inferred from the claim text.