Transgenic plants having altered DAHP synthase activity
AssigneeYeda Research and Development Co. Ltd.
InventorsGad Galili, Asaph Aharoni, Vered Tzin, Sergey Malitsky, Ilana Rogachev
Life of this patent
Estimating the term…
Abstract
The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants.
Claims13
A transgenic dicot plant comprising at least one plant cell comprising an exogenous polynucleotide encoding E. Coli AroG 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) having the amino acid sequence set forth in SEQ ID NO:
- 2 or SEQ ID NO:
- 4 and having at least a 7-fold increase in phenylalanine or prephenate as compared to the level of said phenylalanine or of said prephenate in a transgenic dicot plant expressing wild type E. coli AroG DAHPS set forth by SEQ ID NO:1, or as compared to a wild type control dicot plant of the same type.
A method of modifying a flavor of fruit of a dicot plant, comprising (a) transforming a dicot plant cell with an exogenous polynucleotide encoding E. Coli AroG DAHPS having the amino acid sequence set forth in SEQ ID NO:4, and (b) regenerating the transformed cell into a transgenic dicot plant, wherein the transformed dicot plant has at least a 7-fold increase in phenylalanine or prephenate as compared to the level of said phenylalanine or of said prephenate in a transgenic dicot plant expressing wild type E. coli AroG DAHPS set forth by SEQ ID NO:1, or as compared to a wild type control dicot plant of the same type, and wherein said amino acid sequence modifies a flavor of a fruit as compared to said flavor of said fruit of a wild type control dicot plant of the same type.
A transgenic dicot crop plant comprising at least one plant cell comprising an exogenous polynucleotide encoding E. Coli AroG 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) having the amino acid sequence set forth in SEQ ID NO:
- 4, wherein the transformed dicot crop plant has at least a 7-fold increase in phenylalanine or prephenate as compared to the level of said phenylalanine or of said prephenate in a transgenic dicot crop plant expressing wild type E. coli AroG DAHPS set forth by SEQ ID NO:1, or as compared to a wild type control dicot crop plant of the same type, and wherein said amino acid sequence modifies a flavor of a fruit as compared to said flavor of said fruit of a wild type control dicot crop plant of the same type.
A method of increasing production of a benzeniod phenylpropanoid volatile compound in a crop plant, comprising:
- (a) transforming a dicot plant cell with an exogenous polynucleotide encoding E. Coli AroG DAHPS having the amino acid sequence set forth in SEQ ID NO:
- 2 or SEQ ID NO:
- 4 wherein said amino acid sequence set forth in SEQ ID NO:
- 2 or SEQ ID NO:4 increases production of said benzeniod phenylpropanoid volatile compound as compared to said production of said benzeniod phenylpropanoid volatile compound in a wild type control dicot plant of the same type, and
- (b) regenerating the transformed cell into a transgenic crop plant;
- wherein the transgenic dicot crop plant has at least a 7-fold increase in phenylalanine or prephenate as compared to the level of said phenylalanine or of said prephenate in a transgenic dicot crop plant expressing wild type E. coli AroG DAHPS set forth by SEQ ID NO:
- 1, or as compared to a wild type control dicot crop plant of the same type.
Dependencies are inferred from the claim text.