Brassica AHAS genes and gene alleles that provide resistance to imidazolinone herbicides
OwnerKening Yao, Derek A. Potts +2inventors
InventorsKening Yao, Derek A. Potts, Bradley D. Leibel, Daryl R. Males
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Abstract
Plants, plant parts and plant seeds that are resistant to imidazolinone herbicides are provided. Plants are disclosed that contain a mutation in an AHAS gene. Specifically, plants are disclosed that contain a mutant AHAS gene allele of the Brassica juncea B genome. Two B. juncea AHAS gene sequences (BjAHAS-a and BjAHAS-b) and one B. nigra AHAS gene sequence (BngrAHAS) are disclosed. The sequence of the mutant allele, BjAHAS-bR, is also disclosed. Various methods are disclosed that include creation of mutant B. juncea lines, selection for herbicide resistant lines and determining the presence of the BjAHAS-bR mutant allele after crosses.
Claims13
A method for identifying a plant or seed comprising a mutagenized Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid encoding an herbicide resistance polypeptide comprising an amino acid sequence of residues 114 to 655 of SEQ ID NO:15, said method comprising:
- (A) providing biological material from a plant obtained by a process comprising crossing a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324, with another Brassica variety, or seed thereof,
- (B) performing PCR or hybridization testing of an AHASL gene in said biological material to determine if the biological material comprises the nucleic acid encoding the herbicide resistance polypeptide, and
- (C) identifying, based on the results of step (B), that the plant or seed of step (A) comprises said nucleic acid.
A method for identifying a plant or seed comprising a mutagenized Brassica juncea acetohydroxyacid synthase (AHAS) nucleic acid comprising a sequence having at least 95% homology to SEQ ID NO:14 and encoding an herbicide resistance polypeptide that comprises the Ser to Asn substitution encoded by nucleotides 1968-1970 of SEQ ID NO:14, said method comprising:
- (A) providing biological material from a plant obtained by a process comprising crossing a plant of line J04E-0044, a representative sample of seed of the line having been deposited under ATCC Accession No. PTA-6324, with another Brassica variety, or seed thereof,
- (B) performing PCR or hybridization testing of an AHASL gene in said biological material to determine if the biological material comprises the nucleic acid encoding the herbicide resistance polypeptide, and
- (C) identifying, based on the results of step (B), that the plant or seed of step (A) comprises said nucleic acid.
Dependencies are inferred from the claim text.