Agarooligosaccharide hydrolase and method for producing 3,6-anhydro-L-galactose and galactose from agarose by using same
AssigneeKorea University Research and Business Foundation
InventorsKyoung Heon Kim, In-Geol Choi, Chan Hyoung Lee, Hee Taek Kim, Eun-Ju Yun
Life of this patent
Estimating the term…
Abstract
The present invention relates to agarooligosaccharide hydrolase and a method for producing 3,6-anhydro-L-galactose and galactose from agarose by using the same. More specifically, the production yield of 3,6-anhydro-L-galactose and galactose from agarose, that is, the saccharification yield, is improved by using β-agarooligosaccharide hydrolase having an agarotriose hydrolytic activity.
Claims6
A method of saccharifying agarose to produce 3,6-anhydro-L-galactose and D-galactose comprising:
- reacting acid-pretreated agarose with an enzyme mixture of an agarase, a β-agarooligosaccharide hydrolase, and an α-neoagarobiose hydrolase,
- wherein the agarase comprises the amino acid sequence of SEQ ID NO:
- 3, the β-agarooligosaccharide hydrolase comprises the amino acid sequence of SEQ ID NO:
- 1, and α-neoagarobiose hydrolase comprises the amino acid sequence of SEQ ID NO:
- 4.
A method of producing galactose comprising:
- reacting a substrate with a β-agarooligosaccharide hydrolase to produce galactose,
- wherein the substrate is selected from the group consisting of agarotriose, agaropentaose, and agaroheptaose, and
- wherein the β-agarooligosaccharide hydrolase comprises the amino acid sequence of SEQ ID NO:
- 1.
A method of saccharifying agarose to produce 3,6-anhydro-L-galactose and D-galactose comprising
- allowing an agarase, a β-agarooligosaccharide hydrolase, and an α-neoagarobiose hydrolase to react sequentially with acid-pretreated agarose,
- wherein the agarase comprises the amino acid sequence of SEQ ID NO:
- 3, the β-agarooligosaccharide hydrolase comprises the amino acid sequence of SEQ ID NO:
- 1, and α-neoagarobiose hydrolase comprises the amino acid sequence of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.