Methods and systems for chemoautotrophic production of organic compounds
AssigneeGinkgo Bioworks, Inc.
InventorsCurt R. Fischer, Austin J. Che, Reshma P. Shetty, Jason R. Kelly
Life of this patent
Estimating the term…
Abstract
The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.
Claims6
An engineered cell comprising:
- an energy conversion pathway comprising a formate dehydrogenase having an amino acid sequence of any one of SEQ ID NOs:
- 5-8, or an amino acid sequence encoded by any one of SEQ ID NOs:
- 1-4;
- a carbon fixation pathway, wherein the carbon fixation pathway is a ribulose monophosphate cycle comprising a hexulose-6-phosphate synthase having Enzyme Classification No. E.C. 4.1.2.43;
- and
- a lysine biosynthetic pathway comprising aspartate aminotransferase.
Dependencies are inferred from the claim text.