US 9,902,969 B2Granted

Vector comprising gene fragment for enhancement of recombinant protein expression and use thereof

AssigneeKorea Research Institute of Bioscience and Biotechnology

InventorsEun Gyo Lee, Hong-Weon Lee, Shin Young Kang, Yeon-Gu Kim, Joon Ki Jung, Jungoh Ahn, Seung Hee Kang, Chun Sug Kim, Hyeok Won Lee, Jin Gyeom Lee

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Abstract

The present invention relates to a new gene fragment derived from Chinese hamster ovary (CHO) cell for enhancement of recombinant protein expression in animal cells and a use thereof. It has been found that using the vector comprising a gene fragment of the present invention enhances the expression of a target protein in animal cells. Accordingly, the vector comprising a gene fragment of the present invention could be usefully used in the production of biopharmaceuticals such as therapeutic antibodies, etc.

Claims5

Dependent claims

A recombinant vector for increasing expression of a target gene, comprising a gene fragment consisting of a base sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO:
2, SEQ ID NO:
3, and SEQ ID NO:
4.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 15/319,273Filed 16 Jun 2015
Priority: KR 10-2014-007344317 Jun 2014
Published: US 2017/0218392 A13 Aug 2017
Granted: 27 Feb 2018
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells
C07K 14/43595
From invertebrates: from coelenteratae, e.g. medusae
C07K 16/00
Immunoglobulins [ig]
C12N 15/63
Recombinant DNA-technology: introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression