DGAT genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
AssigneeE. I. Du Pont de Nemours and Company
InventorsHoward Glenn Damude, William D Hitz, Knut Meyer, Narendra S Yadav
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Abstract
Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.
Claims12
A transgenic soybean seed having increased total fatty acid content of at least 10% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed, wherein said seed comprises a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, and wherein said at least one DGAT sequence comprises the nucleic acid sequence encoding a polypeptide having at least 95% sequence identity to the polypeptide having the amino acid sequence set forth in SEQ ID NO:65.
A transgenic soybean seed having an increased total fatty acid content of at least 10% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed, wherein the percentage of fatty acids in said soybean seed that are represented by linolenic acid is decreased, and wherein said seed comprises a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, and wherein said at least one DGAT sequence comprises the nucleic acid sequence encoding a polypeptide having at least 95% sequence identity to the polypeptide having the amino acid sequence set forth in SEQ ID NO:65.
A method for increasing the total fatty acid content of a soybean comprising:
- a) transforming at least one soybean cell with a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, wherein said at least one DGAT sequence comprises the nucleic acid sequence encoding a polypeptide having at least 95% sequence identity to the polypeptide having the amino acid sequence set forth in SEQ ID NO:65;
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- b) selecting the transformed soybean cell(s) of step(a) having an increased total fatty acid content of a non-transgenic, null segregant soybean seed, wherein the percentage of fatty acids in said soybean seed that are represented by linolenic acid is decreased.
Dependencies are inferred from the claim text.