Glycosyltransferase derived from Dolwoe and use thereof
AssigneesKorea Advanced Institute of Science and Technology, Intelligent Synthetic Biology Center
InventorsSun-Chang Kim, Giltsu Choi, Suk-Chae Jung, Woohyun Kim, Soohwan Lim, Wan-Taek Im
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Abstract
Provided are a novel UDP-glycosyltransferase (uridine diphosphate glycosyltransferase) protein having glycosyltransfer activity for glucose linked by a glycosidic bond at the C-20 position of PPD (protopanaxadiol)-type or PPT (protopanaxatriol)-type ginsenoside, and use thereof.
Claims12
A method of preparing a glycosylated protopanaxadiol (PPD)-type ginsenoside or a glycosylated protopanaxatriol (PPT)-type ginsenoside from a PPD-type ginsenoside or a PPT-type ginsenoside, wherein the glycosylated PPT-type ginsenoside and the PPD-type ginsenoside have a glucose that is linked by a glyosidic bond at the C-20 position, the method comprising:
- contacting the PPD-type ginsenoside or the PPT-type ginsenoside and UDP-glucose with:
- (i) an isolated Uridine diphosphate (UDP)-glycosyltransferase protein comprising an amino acid sequence that is at least 95% identical to the sequence of SEQ ID NO:
- 1, and which has activity for transferring a glucose moiety of UDP-glucose to the C-20 position of the PPD-type ginsenoside or the PPT-type ginsenoside, or
- (ii) an isolated host cell transformed with an expression vector, wherein the expression vector comprises a polynucleotide encoding a UDP-glycosyltransferase protein comprising an amino acid sequence that is at least 95% identical to the sequence of SEQ ID NO:
- 1, and which has glycosyltransfer activity for transferring a glucose moiety of UDP-glucose to the C-20 position of the PPD-type ginsenoside or the PPT-type ginsenoside or
- (iii) a culture comprising the host cell of (ii),
- to thereby prepare the glycosylated PPD-type ginsenoside or the glycosylated PPT-type ginsenoside.
An expression vector comprising a polynucleotide encoding a UDP-glycosyltransferase protein and a heterologous promoter operably linked to the polynucleotide, wherein the UDP-glycosyltransferase protein comprises an amino acid sequence that is at least 95% identical to the sequence of SEQ ID NO:
- 1, and which has glycosyltransfer activity for transferring a glucose moiety of UDP-glucose to the C-20 position of a PPD-type ginsenoside or a PPT-type ginsenoside.
Dependencies are inferred from the claim text.