Somatic cell nuclear transfer methods
AssigneeNew York Stem Cell Foundation, Inc.
InventorDietrich M. Egli
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for making reconstructed diploid human oocytes comprising the diploid genome of a human somatic cell, and also methods for making human nuclear transfer embryos, human embryonic stem cells, and human differentiated cells therefrom. The present invention also provides reconstructed human oocytes, human nuclear transfer embryos, human embryonic stem cells, and differentiated cells made using such methods, as well as compositions and kits useful in performing such methods.
Claims10
A method for producing a diploid human nuclear transfer embryo capable of developing into a blastocyst containing an inner cell mass, the method comprising:
- a) obtaining a diploid nuclear genome from a postnatal human somatic cell, obtaining an enucleated mature human oocyte,
- b) transferring the diploid nuclear genome into the enucleated mature human oocyte to form a reconstructed oocyte, wherein the transferring is performed in a medium that is calcium-free, and/or contains a calcium chelator,
- c) subsequently contacting the reconstructed oocyte with a calcium ionophore, an inhibitor of translation, and an inhibitor of meiotic kinases, to activate the reconstructed oocyte and promote entry into interphase, and
- d) contacting the reconstructed oocyte with a histone deacetylase inhibitor and/or a histone methylation inhibitor,
- thereby producing a diploid human nuclear transfer embryo capable of developing into a blastocyst containing an inner cell mass.
Dependencies are inferred from the claim text.