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US 9,896,538 B2Granted
Polyfluoreno[4,5-cde]oxepine polymers and conjugates thereof
AssigneesAAT Bioquest, Inc., Affymetrix, Inc.
InventorsZhenjun Diwu, Haitao Guo, Ruogu Peng, Qin Zhao, Travis Jennings, Castle Funatake
Life of this patent
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Abstract
The invention provides for polyfluoreno[4,5-cde]oxepine conjugates and their use in methods of analyte detection.
Claims26
A polymer comprising monomers
- of formula A
- wherein X is the number of monomer A units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein X is from 6 to 100;
- and one or more monomers selected from the group consisting of
- monomer of formula B
- wherein Y is the number of monomer B units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein Y is from 0 to 99;
- and
- monomer of formula C
- wherein Z is the number of monomer C units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein Z is from 0 to 99;
- wherein R1 to R6 independently is hydrogen, an alkyl, a polyethyleneglycol (PEG), an aryl, a heteroaryl group, or a biological substrate conjugated via a linker (L-BS);
- wherein SG1 to SG6 independently is an alkyl, a water soluble group or a L-BS;
- and
- wherein the polymer includes end groups HG1 and HG2 wherein HG1 and HG2 independently is a hydrogen, an alkyl, a halogen, a boronyl, an aryl, a heteroaryl group or a L-BS;
- wherein the ratio of X to Y+Z is >1,
- wherein the sum of X+Y+Z is >10.
11 more elements
A polymer comprising monomers
- of formula D
- wherein W is the number of monomer D units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein W is from 6 to 100;
- and one or more monomers selected from the group consisting of
- monomer of formula E
- wherein X is the number of monomer E units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein X is from 0 to 98;
- monomer of formula F
- wherein Y is the number of monomer F units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein Y is from 1 to 20;
- and
- monomer of formula G
- wherein Z is the number of monomer G units in the polymer wherein the monomer units are consecutive or nonconsecutive and wherein Z is from 0 to 98;
- and
- wherein R1 to R6 independently is hydrogen, an alkyl, a polyethyleneglycol (PEG), an aryl, a heteroaryl group, or a biological substrate conjugated via a linker (L-BS);
- wherein SG1 to SG7 independently is an alkyl, a water soluble group or a L-BS;
- and
- wherein the polymer includes end groups HG1 and HG2 wherein HG1 and HG2 independently is a hydrogen, an alkyl, a halogen, a boronyl, an aryl, a heteroaryl group or a L-BS;
- wherein the ratio of W to X+Y+Z is >1,
- wherein the sum of W+X+Y+Z is >10,
- wherein FP is a fluorophore or fluorescent dye that has absorption maximum longer than 450 nm and emission maximum longer than 500 nm with fluorescence quantum yield greater than 5%.
15 more elements
Dependencies are inferred from the claim text.