Kits suitable for use in electrochemiluminescence methods and methods of effecting a specific-binding non-wash assay

A kit suitable for use in an electrochemiluminescence method comprising, one or more containers configured to receive a sample, in said one or more containers, an electrode, wherein said electrode comprises a binding domain of a binding reagent immobilized thereto, said binding domain configured to form a complex with a peptide or a protein of said sample, a pH buffer, wherein said pH buffer has a concentration of less than 5 mM inorganic phosphate and comprises glycylglycine or a combination of glycylglycine and tris[hydroxymethyl]aminomethane, an Ru- or Os-containing ECL label and an ECL co-reactant, said composition being substantially free of inorganic phosphate, and wherein said electrode is configured to contact said pH buffer and said ECL co-reactant and said electrode is configured to apply electrochemical energy to said sample, ECL co-reactant and said pH buffer.

A kit suitable for use in an electrochemiluminescence method comprising, one or more containers configured to receive a sample, in said one or more containers, an electrode, wherein said electrode comprises a binding domain of a binding reagent immobilized thereto, said binding domain configured to form a complex with a peptide or a protein of said sample, a pH buffer and tripropylamine, wherein said pH buffer has a concentration of less than 5 mM inorganic phosphate and comprises glycylglycine or a combination of glycylglycine and tris[hydroxymethyl]aminomethane, said composition being substantially free of inorganic phosphate, and wherein said electrode is configured to contact said pH buffer and said tripropylamine and said electrode is configured to apply electrochemical energy to said sample, said pH buffer and said tripropylamine.

A kit suitable for use in an electrochemiluminescence method comprising, one or more containers configured to receive a sample, in said one or more containers:(a) an electrode, wherein said electrode comprises a binding domain of a binding reagent immobilized thereto, said binding domain configured to form a complex with a peptide or a protein of said sample (b) tripropylamine having a concentration ranging from 50 to 1000 mM and (c) a pH buffer having a concentration ranging from 50 to 1000 mM, said pH buffer has a concentration of less than 5 mM inorganic phosphate and comprises glycylglycine or a combination of glycylglycine and tris[hydroxymethyl]aminomethane, and wherein said electrode is configured to contact said pH buffer and said tripropylamine and said electrode is configured to apply electrochemical energy to said sample, said pH buffer and said tripropylamine.

A method of effecting a specific-binding assay, either qualitatively or quantitatively, in a composition comprising:

• (a) a pH buffer having a concentration of less than 5 mM inorganic phosphate and comprises glycylglycine or a combination of glycylglycine and tris[hydroxymethyl]aminomethane (b) a phosphor-specific antibody and (c) an ECL label.

A method of effecting a specific-binding non-wash assay, either qualitatively or quantitatively, in a well having one or more assay domains with binding reagents immobilized thereon using a composition comprising a pH buffer and an ECL label, wherein said pH buffer has a concentration of less than 5 mM inorganic phosphate and comprises glycylglycine or a combination of glycylglycine and tris[hydroxymethyl]aminomethane.