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US 9,890,406 B2Granted
Method for producing cathine
AssigneeForschungszentrum Juelich GmbH
InventorsDoerte Rother, Martina Pohl, Torsten Sehl, Alvaro Gomez Baraibar
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Abstract
A method for producing cathine ((1S,2S)-norpseudoephedrine), in which, in a first reaction step, benzaldehyde is reacted with an acetyl donor according to formula (1), where R═H or COOH, by way of an (S)-selective lease to yield an enantiomer mixture according to formulas (2) and (3) and, in a second step, the compound according to formula (3) is reacted with an amine donor by way of an (S)-selective transaminase to yield (1S,2S)-norpseudoephedrine.
Claims11
A method for producing (1S,2S)-norpseudoephedrine, comprising:
- a first step of reacting benzaldehyde with an acetyl donor according to formula (1)
- where R=H or COOH
- in the presence of an (S)-selective lyase to yield an enantiomer mixture comprising compounds according to formulas (2) and (3)
- and
- a second step of reacting the compound according to formula (3) with an amino donor in the presence of an (S)-selective transaminase to yield (1S,2S)-norpseudoephedrine,
- wherein
- in the first step, a magnesium salt and thiamine diphosphate are used as cofactors, and E469G variant of Acetobacter pasteurianus pyruvate decarboxylase (ApPDC-E469G) is used as the (S)-selective lyase, and
- in the second step, pyridoxal 5′-phosphate is used as a cofactor, and CV2025 from Chromobacterium violaceum is used as the (S)-selective transaminase.
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Dependencies are inferred from the claim text.