Modified isoprene synthase
AssigneeAjinomoto Co., Inc.
InventorsKunio Nakata, Yoshinori Tajima, Uno Tagami, Takashi Oku, Yasuhiro Kashima
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Abstract
The present application relates to a modified isoprene synthase that has an isoprene synthetic activity and has at least one mutation of an amino acid residue in the amino acid sequence of SEQ ID NO: 4, an amino acid sequence having one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO: 4, or an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO: 4. The modified isoprene synthase is useful for preparing isoprene monomers in improved yields.
Claims9
A modified isoprene synthase, having an amino acid sequence at least 90 identical to the amino acid sequence of SEQ ID NO:
- 4,
- wherein the modified isoprene synthase has at least one mutation of an amino acid residue corresponding to at least one amino acid residue in SEQ ID NO:
- 4 selected from the group consisting of:
- C286, E321, C370, A390, E471, C480, I518, and C521, and
- the modified isoprene synthase has an isoprene synthetic activity.
A modified isoprene synthase, having an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:
- 2,
- wherein the modified isoprene synthase has at least one mutation of an amino acid residue corresponding to at least one amino acid residue in SEQ ID NO:
- 2 selected from the group consisting of:
- C329, E364, C413, A433, E514, C523, I561, and C564, and
- the modified isoprene synthase has an isoprene synthetic activity.
Dependencies are inferred from the claim text.