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US 9,885,705 B2Granted
Cell analysis using dynamic biophysical methods
AssigneeBar-Ilan University
InventorsYuval Garini, Irena Bronshtein Berger, Eldad Kepten
Life of this patent
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Abstract
A method for identifying the expression or activity of a nuclear protein in a cell, the method comprising: analyzing multiple images of one or more labeled genetic entities; determining from said analysis, a motile property of said one or more labeled genetic entities; and identifying an expression or activity of a nuclear protein of said cell associated with said motile property.
Claims9
A method for determining the ratio of bound to unbound molecules in a cell, comprising:
- obtaining, from a selected area of a cell, data comprising time-resolved emitted light intensity measurements and time-resolved fluorescent lifetime measurements of fluorescently labeled bound molecules and fluorescently labeled unbound molecules;
- determining from the obtained data 1) a continuous photobleaching (CP) curve and 2) fluorescent lifetime histograms for each of the time-resolved emitted light intensity measurements and fluorescent lifetime measurements;
- and
- calculating a ratio of the fluorescently labeled bound molecules to the fluorescently labeled unbound molecules in the selected regions using the CP curve and the fluorescent lifetime histograms.
1 more element
Dependencies are inferred from the claim text.