Chip for analysis of target substance

A system for analysis of a target substance comprising:

• a chip;
• and
• a magnetic field generating unit configured to generate a magnetic field,
• the chip comprising a laminate, the laminate comprising:
• a first flexible substrate;
• a second flexible substrate;
• and
• a third substrate, wherein
• a flow channel-forming non-bonded area is formed on a bonding surface of the first flexible substrate and the second flexible substrate in a band-like manner and an extraction chamber-forming non-bonded area having a wider band width than the flow channel-forming non-bonded area is formed at a part of the flow channel-forming nonbonded area,
• the first flexible substrate comprises a through-hole that is in contact with the flow channel-forming non-bonded area,
• a shutter-forming non-bonded area is formed on a bonding surface of the second flexible substrate and the third substrate in a band-like manner at a far side of the through-hole relative to the extraction chamber-forming non-bonded area such that the shutter-forming non-bonded area and the flow channel-forming non-bonded area intersect via the second flexible substrate being interposed therebetween,
• at least one of both of the first flexible substrate and the second flexible substrate and the third substrate comprises a pressure supply port that comes through the substrate(s) so as to be in contact with the shutter-forming non-bonded area, a magnetic particle that binds to a target substance is placed above the extraction chamber-forming non-bonded area,
• a flow channel and an extraction chamber are formable by supplying pressure from the through-hole to raise a site above the flow channel-forming non-bonded area and a site above the extraction chamber-forming non-bonded area,
• the flow channel is blockable by supplying pressure from the pressure supply port to raise a site above the shutter-forming non-bonded area,
• the magnetic field generating unit is disposed 1) at an outer side of at least one of the first flexible substrate or the third flexible substrate, and 2) in a vicinity of an end of the extraction chamber at the opposite side of the through-hole, and
• the target substance that is bound to the magnetic particle is capturable by the magnetic field at at least one of an undersurface of the third substrate directly below an end of the extraction chamber at the opposite side of the through-hole or a top surface of the first flexible substrate directly above an end of the extraction chamber at the opposite side of the through-hole.