Process for the production of an enzymatic cocktail using solid residues from a process for the biochemical coversion of lignocellulosic materials

A process for the production of an enzymatic cocktail with a cellulolytic microorganism, comprising two phases:

• a phase a) comprising growing said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said phase a) being carried out with a concentration of carbonaceous growth substrate in the range of 10 to 90 g/L;
• and
• a phase b) comprising producing the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied to the reactor, said carbonaceous inducer substrate being at least one solid residue obtained by enzymatic partial hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said solid residue having a liquid portion and a solid portion wherein the solid portion constitutes 10% to 40% by weight of the solid residue and said solid residue having no solid residue obtained from ethanolic fermentation of enzymatic hydrolysates from the enzymatic partial hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said phase b) being carried out with a concentration of carbonaceous inducer substrate in the range of 150 to 400 g/L, wherein the partial hydrolysis is conducted with an initial enzymatic cocktail, wherein, in the partial hydrolysis, only 20% to 70% by weight of the lignocellulosic materials subject to the enzymatic partial hydrolysis are hydrolysed and wherein the partial hydrolysis is conducted with:
• a dry matter content of more than 20% by weight;
• and/or
• with less than 10 mg of the initial enzymatic cocktail per gram of cellulose in the lignocellulosic materials which have undergone a pre-treatment step.