Method and array for identifying histone-code-related analytes
AssigneeUVic Industry Partnerships Inc.
InventorsFraser Hof, Samuel Minaker, Kevin Daze, Sara Tabet, Manuel Ma
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Abstract
Disclosed embodiments concern an array for use in identifying or identifying and quantifying analytes in a sample using a macrocyclic sensor comprising a macrocyclic compound and a detectable moiety. The disclosed array may be used to discriminate among various analytes based on different features, such as post-translational modifications, isomeric post-translational modifications, and the peptide sequence around post-translational modifications. Also disclosed is a method for identifying analytes comprising a post-translational modification, as well as an enzymatic assay using the disclosed macrocyclic sensor.
Claims33
A macrocyclic compound having a formula
- wherein R1 is H, R2 is selected from SO3H, CO2H, and PO3H2, and R3 is heteroaryl, aryl, amine, or combinations thereof.
An array, comprising at least two macrocyclic sensors, wherein at least one macrocyclic sensor comprises a macrocyclic compound having a formula selected from
- or an anionic form thereof in an aqueous environment, wherein:
- R1 is H;
- each R2 is independently selected from SO3H, CO2H, or PO3H2;
- and
- R3 is heteroaryl, aryl, amine, or combinations thereof.
A method, comprising:
- exposing a sample comprising an analyte to a sensor comprising a macrocyclic compound having a formula selected from
- or an anionic form thereof in an aqueous environment, wherein R1 is H;
- each R2 is independently selected from SO3H, CO2H, or PO3H2;
- and each R3 is independently selected from heteroaryl, aryl, amine, or combinations thereof;
- and
- detecting a signal produced by interaction between the analyte and the sensor.
A macrocyclic compound having a structure
Dependencies are inferred from the claim text.