US 9,879,280 B2Granted

Baculovirus system for expressing proteins forming virus-like particles

AssigneeAlternative Gene Expression S.L.

InventorsSilvia Gomez Sebastian, Javier López Vidal, José Angel Martinez Escribano

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention may be included in the field of biotechnology and it covers the improved production of recombinant proteins in insect cells or insect larvae as biofactories by a novel expression cassette. This expression cassette comprises nucleic acid sequences such as promoters, homologous regions (hr) as enhancers, and sequences encoding transcriptional regulators, for example, the baculovirus Ac-ie-01 cDNA, or any combination thereof, which are able to increase the quality and production efficiency of the recombinant proteins. Moreover, the present invention is also directed to the vectors themselves comprising the above mentioned nucleic acid sequences of the invention, cells or insects infected, transformed or transfected with those sequences or vectors, and methods for producing the recombinant proteins by using the aforesaid sequences, vectors, cells or insects.

Claims12

Dependent claims

An expression cassette for a baculovirus expression vector system (BEVS), comprising a first nucleic acid sequence and a second nucleic acid sequence;

wherein the first nucleic acid sequence is selected from the group consisting of:
(a) a nucleic acid sequence comprising the sequence of SEQ ID NO:
1;
(b) a nucleic acid sequence having at least 95% sequence identity to the sequence of SEQ ID NO:1 and encoding a protein able to function as a transcriptional regulator in a baculovirus;
(c) a nucleic acid sequence encoding an amino acid sequence comprising the sequence of SEQ ID NO:
6;
and
(d) a nucleic acid sequence encoding a protein able to function as a transcriptional regulator in a baculovirus and having at least 95% sequence identity to the sequence of SEQ ID NO:
6;
wherein the second nucleic acid sequence directs the expression of a recombinant protein, wherein the recombinant protein is a virus-like particle protein;
wherein the expression cassette for BEVS also comprises at least one recombinant homologous region (hr) as enhancer region operably linked to a promoter suitable for driving the expression of the recombinant protein;
wherein the promoter suitable for driving the expression of the recombinant protein comprises a nucleic acid sequence selected from the group consisting of:
(a) a nucleic acid sequence comprising the sequence of SEQ ID NO:13;
and
(b) a nucleic acid sequence able to function as a promoter in a baculovirus and having at least 95% sequence identity to the sequence of SEQ ID NO:13.

12 more elements

Dependencies are inferred from the claim text.