High activity mutants of cocaine esterase for cocaine hydrolysis
AssigneeUniversity of Kentucky Research Foundation
InventorsChang-Guo Zhan, Fang Zheng, Lei Fang
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Abstract
The Bacterial cocaine esterase (CocE) mutants disclosed herein each have enhanced catalytic efficiency for (−)-cocaine, as compared to CocE mutants in the prior art, including CocE mutant E172-173. The presently-disclosed subject matter further includes a pharmaceutical composition including a mutant of bacterial cocaine hydrolase, as described herein, and a suitable pharmaceutical carrier. The presently-disclosed subject matter further includes a method of treating a cocaine-induced condition comprising administering to an individual an effective amount of a mutant of bacterial cocaine hydrolase variant, as disclosed herein, to accelerate cocaine metabolism and produce biologically inactive metabolites.
Claims14
A cDNA molecule comprising the sequence of SEQ ID NO:
- 3.
A cDNA molecule comprising a nucleic acid sequence which encodes a cocaine esterase (CocE) polypeptide variant, comprising the amino acid sequence of SEQ ID NO:
- 4.
A cocaine esterase (CocE) polypeptide variant, comprising the amino acid sequence of SEQ ID NO:
- 2, including the amino acid sequences mutations:
- T172R, G173Q, L196C, and 1301C.
A cocaine esterase (CocE) polypeptide variant, comprising the amino acid sequence of SEQ ID NO:
- 4, or the amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.