Brassica ROD1 gene sequences and uses thereof
AssigneeBayer CropScience AG
InventorsPeter Denolf, Michel Van Thournout, Stephane Bourot
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Abstract
The present invention relates to Brassica juncea ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.
Claims8
A Brassica juncea plant, or a cell, part, seed or progeny thereof, comprising seven ROD1 genes, characterized in that two ROD1 genes are knock-out ROD1 genes, wherein one knock-out ROD1 gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2, and wherein one knock-out ROD1 gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4.
A transgenic Brassica juncea plant comprising seven ROD1 genes, or a cell, part, seed or progeny thereof, comprising a chimeric gene, said chimeric gene comprising the following operably linked DNA fragments:
- a) a plant-expressible promoter;
- b) a DNA region, which when transcribed yields an RNA molecule inhibitory to at least two ROD1 genes;
- and optionally
- c) a transcription termination and polyadenylation region functional in plant cells, wherein said RNA molecule is inhibitory to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2 and to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4.
A method for increasing the levels of C 18:1 and/or decreasing the levels of saturated fatty acids in seed oil of a Brassica juncea plant comprising seven ROD1 genes, comprising the steps of:
- a) introducing or providing a chimeric gene to a Brassica juncea plant cell, to create transgenic cells, said chimeric gene comprising the following operably linked DNA fragments:
- a plant-expressible promoter
- a DNA region, which when transcribed yields an RNA molecule inhibitory to at least two ROD1 genes;
- and optionally
- a transcription termination and polyadenylation region functional in plant cells;
- and
- b) regenerating transgenic plants from said transgenic cells wherein said RNA molecule is inhibitory to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2 and to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4.
A method for increasing the levels of C 18:1 and/or decreasing the levels of saturated fatty acids in seed oil of a Brassica juncea plant comprising seven ROD1 genes, comprising the steps of:
- a) treating seeds or plant material with a mutagenic chemical substance or with ionizing radiation;
- b) identifying plants with two mutated ROD1 genes, wherein one ROD1 gene, prior to being mutated, encodes a polypeptide having at least 90% sequence identity to SEQ ID No. 2, and wherein one ROD1 gene, prior to being mutated, encodes a polypeptide having at least 90% sequence identity to SEQ ID No. 4;
- and
- c) selecting a plant with an increased level of C18:1 in the seeds compared to a plant in which the ROD1 gene is not mutated.
Dependencies are inferred from the claim text.