# US 9,873,119 B2 · Multiple- analyte assay device and system

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- Number: US 9,873,119 B2
- Document ID: US9873119B2
- Status: Granted
- Kind: B2
- Application number: 14/369,632
- Filed: 2012-12-28
- Published: 2015-01-08
- Granted: 2018-01-23
- Earliest claimed: 2011-12-28
- Assignees: Ibis Biosciences, Inc.
- Applicants: Ibis Biosciences, Inc.
- Inventors: Mark W. Eshoo; John Picuri
- Web page: https://patentgrep.com/patent/US9873119B2

## CPC

- B01L 3/502769: {characterised by multiphase flow arrangements}
- B01L 7/00: Heating or cooling apparatus (autoclaves B01J3/04); Heat insulating devices
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- B01L 2200/06: Fluid handling related problems
- B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- G01N 2035/00158: {Elements containing microarrays, i.e. "biochip"}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Provided herein are systems and methods for performing complex chemical, physical and biological assays. In particular, provided herein are systems and methods for performing microfluidic assays.

## Claims

### Claim 1

A system comprising:
a) a cartridge comprising:
i) a central reaction vessel; and
ii) a plurality of auxiliary vessels radially linked in direct fluid communication with said central reaction vessel, wherein said auxiliary vessels comprise reagents for performing an assay, and wherein said assay detects an analyte without transferring said sample from said central reaction vessel; and
b) an external source of environmental control in communication with said cartridge wherein said external source of environmental control is an ultrasonic transducer.

### Claim 2 (depends on 1)

The system of claim 1, wherein said cartridge further comprises a sample input and/or a sample output component in fluid communication with said central reaction vessel.

### Claim 3 (depends on 1)

The system of claim 1, wherein said assay is selected from the group consisting of an enzymatic assay, a chemical assay, a biochemical assay, an immunological assay, a physical assay, and a biological assay.

### Claim 4 (depends on 1)

The system of claim 1, wherein said analyte is selected from the group consisting of a chemical, a nucleic acid, a polypeptide, a lipid, a carbohydrate, and combinations thereof.

### Claim 5 (depends on 1)

The system of claim 1, wherein said external source of environmental control is selected from the group consisting of a heater/cooler, and a light source.

### Claim 6 (depends on 1)

The system of claim 1, wherein said system further comprises a plurality of sample preparation components in fluid communication with said cartridge.

### Claim 7 (depends on 6)

The system of claim 6, wherein said sample preparation components are selected from the group consisting of cell lysis components, nucleic acid extraction components, sample purification components, and analyte capture components.

### Claim 8 (depends on 1)

The system of claim 1, wherein said system comprises a plurality of said cartridges.

### Claim 9 (depends on 1)

The system of claim 1, wherein said system further comprises a plurality of sample detection or analysis components.

### Claim 10 (depends on 9)

The system of claim 9, wherein said sample detection or analysis components comprise sensors.

### Claim 11 (depends on 1)

A method of performing an assay, the method comprising:
a) contacting a sample with the system of claim 1; and
b) performing an assay with said system.

### Claim 12 (depends on 11)

The method of claim 11, further comprising detecting the presence or absence of an analyte in said sample using one or more sample detection or analysis components.

### Claim 13 (depends on 11)

The method of claim 11, wherein said assay is selected from the group consisting of an enzymatic assay, a chemical assay, a biochemical assay, a physical assay, and a biological assay.

### Claim 14 (depends on 12)

The method of claim 12, wherein said analyte is selected from the group consisting of a chemical, a nucleic acid, a polypeptide, a lipid, a carbohydrate, and combinations thereof.

### Claim 15 (depends on 11)

The method of claim 11, wherein said sample is selected from the group consisting of a biological sample, an environmental sample, a cellular sample, and chemical sample.

### Claim 16 (depends on 15)

The method of claim 15, wherein said cellular sample comprises a virus, a bacterium, a fungus, and/or a eukaryotic cell.